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PDBsum entry 4f10
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PDB id:
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Lyase
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Title:
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Alginate lyase a1-iii h192a complexed with tetrasaccharide
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Structure:
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Alginate lyase. Chain: a, b. Fragment: unp residues 54-404. Synonym: alginate lyase a1-iii. Engineered: yes. Mutation: yes
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Source:
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Sphingomonas. Organism_taxid: 28214. Strain: a1. Gene: aly. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
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Resolution:
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2.20Å
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R-factor:
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0.196
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R-free:
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0.239
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Authors:
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B.Mikami,M.Ban,S.Suzuki,H.-J.Yoon,O.Miyake,M.Yamasaki,K.Ogura, Y.Maruyama,W.Hashimoto,K.Murata
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Key ref:
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B.Mikami
et al.
(2012).
Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III.
Acta Crystallogr D Biol Crystallogr,
68,
1207-1216.
PubMed id:
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Date:
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05-May-12
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Release date:
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27-Jun-12
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Supersedes:
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PROCHECK
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Headers
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References
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Q9KWU1
(Q9KWU1_SPHSX) -
Alginate lyase from Sphingomonas sp
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Seq:
Struc:
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641 a.a.
353 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 4 residue positions (black
crosses)
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Enzyme class:
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E.C.4.2.2.3
- mannuronate-specific alginate lyase.
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Reaction:
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Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4- enopyranuronosyl groups at their ends.
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Acta Crystallogr D Biol Crystallogr
68:1207-1216
(2012)
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PubMed id:
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Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III.
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B.Mikami,
M.Ban,
S.Suzuki,
H.J.Yoon,
O.Miyake,
M.Yamasaki,
K.Ogura,
Y.Maruyama,
W.Hashimoto,
K.Murata.
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ABSTRACT
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Links
