PDBsum entry 4est

Hydrolase/hydrolase inhibitor

PDB id: 4est

Contents

Protein chain

240 a.a. *

Ligands

ACE-ALA-PRO-VAI-FPA-PEA

SO4

Metals

_CA

Waters ×156

* Residue conservation analysis

PDB id:

4est

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of the covalent complex formed by a peptidyl alpha, alpha-difluoro-beta-keto amide with porcine pancreatic elastase at 1.78-angstroms resolution

Structure:

Elastase. Chain: e. Engineered: yes. Inhibitor ace-ala-pro-vai-difluoro-n-phenylethylacetamide. Chain: i. Engineered: yes

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Tissue: pancreas. Synthetic: yes

Biol. unit:

Dimer (from PQS)

Resolution:

1.78Å R-factor: 0.160

Authors:

L.H.Takahashi,R.Radhakrishnan,R.E.Rosenfieldjunior,E.F.Meyerjunior, D.A.Trainor

Key ref:

L.H.Takahashi et al. (1989). Crystal structure of the covalent complex formed by a peptidyl alpha,Alpha-Difluoro-Beta-Keto amide with porcine pancreatic elastase at 1.78-Angstroms resolution. J am chem soc, 111, 3368.

Date:

15-May-89 Release date: 15-Apr-92

Protein chain

P00772  (CELA1_PIG) -  Chymotrypsin-like elastase family member 1 from Sus scrofa

Seq: 266 a.a.

Struc: 240 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.21.36 - pancreatic elastase.
• Reaction: Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.