PDBsum entry 4esb

Transcription

PDB id: 4esb

Contents

Protein chain

103 a.a.

Ligands

SO4

GOL ×4

Waters ×17

PDB id:

4esb

Name:

Transcription

Title:

Crystal structure of padr-like transcriptional regulator (bc4206) from bacillus cereus strain atcc 14579

Structure:

Transcriptional regulator, padr family. Chain: a. Engineered: yes

Source:

Bacillus cereus. Organism_taxid: 226900. Strain: atcc 14579 / dsm 31. Gene: bc_4206. Expressed in: lactococcus lactis. Expression_system_taxid: 746361.

Resolution:

2.50Å R-factor: 0.202 R-free: 0.226

Authors:

G.Fibriansah,A.T.Kovacs,O.P.Kuipers,A.M.W.H.Thunnissen

Key ref:

G.Fibriansah et al. (2012). Crystal structures of two transcriptional regulators from Bacillus cereus define the conserved structural features of a PadR subfamily. Plos One, 7, e48015. PubMed id: 23189126

Date:

23-Apr-12 Release date: 05-Dec-12

Protein chain

Q818P3  (Q818P3_BACCR) -  Transcriptional regulator, PadR family from Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711)

Seq: 105 a.a.

Struc: 103 a.a.

Plos One 7:e48015 (2012)

PubMed id: 23189126

Crystal structures of two transcriptional regulators from Bacillus cereus define the conserved structural features of a PadR subfamily.

G.Fibriansah, �.�.T.Kovács, T.J.Pool, M.Boonstra, O.P.Kuipers, A.M.Thunnissen.

ABSTRACT

PadR-like transcriptional regulators form a structurally-related family of proteins that control the expression of genes associated with detoxification, virulence and multi-drug resistance in bacteria. Only a few members of this family have been studied by genetic, biochemical and biophysical methods, and their structure/function relationships are still largely undefined. Here, we report the crystal structures of two PadR-like proteins from Bacillus cereus, which we named bcPadR1 and bcPadR2 (products of gene loci BC4206 and BCE3449 in strains ATCC 14579 and ATCC 10987, respectively). BC4206, together with its neighboring gene BC4207, was previously shown to become significantly upregulated in presence of the bacteriocin AS-48. DNA mobility shift assays reveal that bcPadR1 binds to a 250 bp intergenic region containing the putative BC4206-BC4207 promoter sequence, while in-situ expression of bcPadR1 decreases bacteriocin tolerance, together suggesting a role for bcPadR1 as repressor of BC4206-BC4207 transcription. The function of bcPadR2 (48% identical in sequence to bcPadR1) is unknown, but the location of its gene just upstream from genes encoding a putative antibiotic ABC efflux pump, suggests a role in regulating antibiotic resistance. The bcPadR proteins are structurally similar to LmrR, a PadR-like transcription regulator in Lactococcus lactis that controls expression of a multidrug ABC transporter via a mechanism of multidrug binding and induction. Together these proteins define a subfamily of conserved, relatively small PadR proteins characterized by a single C-terminal helix for dimerization. Unlike LmrR, bcPadR1 and bcPadR2 lack a central pore for ligand binding, making it unclear whether the transcriptional regulatory roles of bcPadR1 and bcPadR2 involve direct ligand recognition and induction.