PDBsum entry 4erd

RNA binding protein/RNA

PDB id: 4erd

Contents

Protein chains

108 a.a.

DNA/RNA

Metals

__K

Waters ×22

PDB id:

4erd

Name:

RNA binding protein/RNA

Title:

Crystal structure of thE C-terminal domain of tetrahymena telomerase protein p65 in complex with stem iv of telomerase RNA

Structure:

Telomerase associated protein p65. Chain: a, b. Fragment: see remark 999. Engineered: yes. 5'-r(p Gp Gp Up Cp Gp Ap Cp Ap Up Cp Up Up Cp Gp Gp Ap Up G p Gp Ap Cp C)-3'. Chain: c, d. Engineered: yes. Other_details: stem iv of telomerase RNA

Source:

Tetrahymena thermophila. Organism_taxid: 5911. Gene: tap65. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: RNA was prepared by in vitro transcription with t7 RNA polymerase

Resolution:

2.59Å R-factor: 0.222 R-free: 0.272

Authors:

M.Singh,Z.Wang,B.-K.Koo,A.Patel,D.Cascio,K.Collins,J.Feigon

Key ref:

M.Singh et al. (2012). Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65. Mol Cell, 47, 16-26. PubMed id: 22705372

Date:

19-Apr-12 Release date: 20-Jun-12

Protein chains

W7X6T2  (W7X6T2_TETTS) -  La-related protein 7 homolog from Tetrahymena thermophila (strain SB210)

Seq: 542 a.a.

Struc: 108 a.a.

DNA/RNA chains

G-G-U-C-G-A-C-A-U-C-U-U-C-G-G-A-U-G-G-A-C-C 22 bases

G-G-U-C-G-A-C-A-U-C-U-U-C-G-G-A-U-G-G-A-C-C 22 bases

Mol Cell 47:16-26 (2012)

PubMed id: 22705372

Structural basis for telomerase RNA recognition and RNP assembly by the holoenzyme La family protein p65.

M.Singh, Z.Wang, B.K.Koo, A.Patel, D.Cascio, K.Collins, J.Feigon.

ABSTRACT

No abstract given.