PDBsum entry 4e5m

Oxidoreductase

PDB id: 4e5m

Contents

Protein chains

329 a.a.

Ligands

NAP ×2

Waters ×515

PDB id:

4e5m

Name:

Oxidoreductase

Title:

Thermostable phosphite dehydrogenase e175a/a176r in complex with NADP

Structure:

Thermostable phosphite dehydrogenase. Chain: a, b. Engineered: yes

Source:

Pseudomonas stutzeri. Organism_taxid: 316. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.85Å R-factor: 0.187 R-free: 0.217

Authors:

Y.Zou,H.Zhang,S.K.Nair

Key ref:

Y.Zou et al. (2012). Crystal structures of phosphite dehydrogenase provide insights into nicotinamide cofactor regeneration. Biochemistry, 51, 4263-4270. PubMed id: 22564171

Date:

14-Mar-12 Release date: 30-May-12

Protein chains

O69054  (PTXD_STUST) -  Phosphonate dehydrogenase from Stutzerimonas stutzeri

Seq: 336 a.a.

Struc: 329 a.a.*

* PDB and UniProt seqs differ at 16 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.20.1.1 - phosphonate dehydrogenase.
• Reaction: phosphonate + NAD⁺ + H2O = phosphate + NADH + H⁺

phosphonate + NAD(+) (Bound ligand (Het Group name = NAP) matches with 91.67% similarity) + H2O = phosphate + NADH + H(+)

• Cofactor: Iron-sulfur; Mo cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochemistry 51:4263-4270 (2012)

PubMed id: 22564171

Crystal structures of phosphite dehydrogenase provide insights into nicotinamide cofactor regeneration.

Y.Zou, H.Zhang, J.S.Brunzelle, T.W.Johannes, R.Woodyer, J.E.Hung, N.Nair, W.A.van der Donk, H.Zhao, S.K.Nair.

ABSTRACT

No abstract given.