PDBsum entry 4dqs

Transferase/DNA

PDB id: 4dqs

Contents

Protein chain

573 a.a.

DNA/RNA

Ligands

CTP

SO4 ×2

Waters ×857

PDB id:

4dqs

Name:

Transferase/DNA

Title:

Binary complex of bacillus DNA polymerase i large fragment and duplex DNA with rc in primer terminus paired with dg of template

Structure:

DNA polymerase. Chain: a. Fragment: un residues 287-878. Engineered: yes. Mutation: yes. DNA/RNA (5'-d( Gp Cp Gp Ap Tp Cp Ap Cp Gp Tp A)-r(p C)-3'). Chain: b. Engineered: yes. Other_details: DNA primer strand.

Source:

Geobacillus kaustophilus. Organism_taxid: 235909. Strain: hta426. Gene: pola, gk2730. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic: yes

Resolution:

1.66Å R-factor: 0.161 R-free: 0.181

Authors:

W.Wang,L.S.Beese

Key ref:

W.Wang et al. (2012). Structural factors that determine selectivity of a high fidelity DNA polymerase for deoxy-, dideoxy-, and ribonucleotides. J Biol Chem, 287, 28215-28226. PubMed id: 22648417

Date:

16-Feb-12 Release date: 06-Jun-12

Protein chain

Q5KWC1  (Q5KWC1_GEOKA) -  DNA polymerase I from Geobacillus kaustophilus (strain HTA426)

Seq: 878 a.a.

Struc: 573 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

C-G-A-T-C-A-C-G-T-A-C 11 bases

C-G-T-A-C-G-T-G-A-T-C-G 12 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Biol Chem 287:28215-28226 (2012)

PubMed id: 22648417

Structural factors that determine selectivity of a high fidelity DNA polymerase for deoxy-, dideoxy-, and ribonucleotides.

W.Wang, E.Y.Wu, H.W.Hellinga, L.S.Beese.

ABSTRACT

No abstract given.