PDBsum entry 4dls

Signaling protein

PDB id: 4dls

Contents

Protein chain

166 a.a.

Ligands

GNP

Metals

_CA ×2

_MG ×2

Waters ×109

PDB id:

4dls

Name:

Signaling protein

Title:

H-ras set 1 cacl2 'mixed'

Structure:

Gtpase hras. Chain: a. Synonym: h-ras-1, ha-ras, transforming protein p21, c-h-ras, p21ras, gtpase hras, n-terminally processed. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hras, hras1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.82Å R-factor: 0.163 R-free: 0.194

Authors:

G.Holzapfel,C.Mattos

Key ref:

G.Holzapfel et al. (2012). Shift in the equilibrium between on and off states of the allosteric switch in Ras-GppNHp affected by small molecules and bulk solvent composition. Biochemistry, 51, 6114-6126. PubMed id: 22845804

Date:

06-Feb-12 Release date: 08-Aug-12

Protein chain

P01112  (RASH_HUMAN) -  GTPase HRas from Homo sapiens

Seq: 189 a.a.

Struc: 166 a.a.

Enzyme reactions

• Enzyme class: E.C.3.6.5.2 - small monomeric GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

GTP + H2O = GDP (Bound ligand (Het Group name = GNP) matches with 81.82% similarity) + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochemistry 51:6114-6126 (2012)

PubMed id: 22845804

Shift in the equilibrium between on and off states of the allosteric switch in Ras-GppNHp affected by small molecules and bulk solvent composition.

G.Holzapfel, G.Buhrman, C.Mattos.

ABSTRACT

No abstract given.