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PDBsum entry 4dfp
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Transferase/DNA
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PDB id
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4dfp
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PDB id:
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| Name: |
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Transferase/DNA
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Title:
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Crystal structure of the large fragment of DNA polymerase i from thermus aqauticus in a ternary complex with 7-(aminopentinyl)-7- deaza-dgtp
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Structure:
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DNA polymerase i, thermostable. Chain: a. Fragment: klenow fragment, unp residues 293-831. Synonym: taq polymerase 1. Engineered: yes. DNA (5'-d( Gp Ap Cp Cp Ap Cp Gp Gp Cp Gp Cp (Doc))-3'). Chain: b. Engineered: yes. 5'-d(aaacggcgccgtggtc)-3'.
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Source:
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Thermus aquaticus. Organism_taxid: 271. Gene: pola, pol1. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic: yes
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Resolution:
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2.00Å
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R-factor:
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0.187
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R-free:
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0.224
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Authors:
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K.Bergen,A.Steck,S.Struett,A.Baccaro,W.Welte,K.Diederichs,A.Marx
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Key ref:
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K.Bergen
et al.
(2012).
Structures of KlenTaq DNA polymerase caught while incorporating C5-modified pyrimidine and C7-modified 7-deazapurine nucleoside triphosphates.
J Am Chem Soc,
134,
11840-11843.
PubMed id:
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Date:
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24-Jan-12
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Release date:
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16-May-12
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PROCHECK
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Headers
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References
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P19821
(DPO1_THEAQ) -
DNA polymerase I, thermostable from Thermus aquaticus
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Seq:
Struc:
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832 a.a.
539 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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G-A-C-C-A-C-G-G-C-G-C-DOC
12 bases
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A-A-A-C-G-G-C-G-C-C-G-T-G-G-T-C
16 bases
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Enzyme class:
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E.C.2.7.7.7
- DNA-directed Dna polymerase.
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Reaction:
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DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
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DNA(n)
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+
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2'-deoxyribonucleoside 5'-triphosphate
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=
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DNA(n+1)
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+
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diphosphate
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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J Am Chem Soc
134:11840-11843
(2012)
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PubMed id:
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Structures of KlenTaq DNA polymerase caught while incorporating C5-modified pyrimidine and C7-modified 7-deazapurine nucleoside triphosphates.
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K.Bergen,
A.L.Steck,
S.Strütt,
A.Baccaro,
W.Welte,
K.Diederichs,
A.Marx.
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ABSTRACT
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Links
