PDBsum entry 4aw7

Hydrolase

PDB id: 4aw7

Contents

Protein chain

565 a.a.

Ligands

GAL

EDO

GLA-L6S-GAL-AAL-GAL-L6S

Metals

IOD ×13

_CA

_CL

__K ×2

Waters ×594

PDB id:

4aw7

Name:

Hydrolase

Title:

Bpgh86a: a beta-porphyranase of glycoside hydrolase family 86 from the human gut bacterium bacteroides plebeius

Structure:

Gh86a beta-porphyranase. Chain: a. Fragment: catalytic domain, residues 30-598. Engineered: yes

Source:

Bacteroides plebeius. Organism_taxid: 310297. Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: german collection of microorganisms

Resolution:

1.33Å R-factor: 0.138 R-free: 0.152

Authors:

J.H.Hehemann,A.G.Kelly,N.A.Pudlo,E.C.Martens,A.B.Boraston

Key ref:

J.H.Hehemann et al. (2012). Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes. Proc Natl Acad Sci U S A, 109, 19786-19791. PubMed id: 23150581

Date:

01-Jun-12 Release date: 25-Jul-12

Protein chain

B5CY96  (PORA_PHOPM) -  Beta-porphyranase A from Phocaeicola plebeius (strain DSM 17135 / JCM 12973 / M2)

Seq: 728 a.a.

Struc: 565 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.178 - beta-porphyranase.

Proc Natl Acad Sci U S A 109:19786-19791 (2012)

PubMed id: 23150581

Bacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbes.

J.H.Hehemann, A.G.Kelly, N.A.Pudlo, E.C.Martens, A.B.Boraston.

ABSTRACT

No abstract given.