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PDBsum entry 4am0
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Viral protein
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PDB id
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4am0
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Contents |
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211 a.a.
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212 a.a.
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91 a.a.
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96 a.a.
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PDB id:
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| Name: |
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Viral protein
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Title:
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Structure of dengue virus strain 4 diii in complex with fab 2h12
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Structure:
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Fab 2h12, heavy chain. Chain: a, c, e, h. Engineered: yes. Fab 2h12, light chain. Chain: b, d, f, l. Engineered: yes. Envelope protein,. Chain: q, r, s, t. Fragment: domain iii, residues 295-395.
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Source:
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Homo sapiens, mus musculus. Human, house mouse. Organism_taxid: 9606, 10090. Expressed in: mus musculus. Expression_system_taxid: 10090. Dengue virus. Organism_taxid: 11070. Strain: 4. Expressed in: escherichia coli.
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Resolution:
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3.02Å
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R-factor:
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0.199
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R-free:
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0.252
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Authors:
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C.M.Midgley,A.Flanagan,J.Mongkolsapaya,J.M.Grimes,G.R.Screaton
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Key ref:
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C.M.Midgley
et al.
(2012).
Structural analysis of a dengue cross-reactive antibody complexed with envelope domain III reveals the molecular basis of cross-reactivity.
J Immunol,
188,
4971-4979.
PubMed id:
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Date:
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06-Mar-12
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Release date:
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06-Jun-12
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PROCHECK
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Headers
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References
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No UniProt id for this chain
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No UniProt id for this chain
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Enzyme class 1:
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Chains Q, R, S, T:
E.C.2.1.1.56
- mRNA (guanine-N(7))-methyltransferase.
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Reaction:
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a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L- methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine
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5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA
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+
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S-adenosyl-L- methionine
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=
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5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA
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+
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S-adenosyl-L-homocysteine
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Enzyme class 2:
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Chains Q, R, S, T:
E.C.2.1.1.57
- methyltransferase cap1.
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Reaction:
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a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)- (2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H+
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5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA
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+
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S-adenosyl-L-methionine
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=
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5'-end (N(7)-methyl 5'-triphosphoguanosine)- (2'-O-methyl-ribonucleoside) in mRNA
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+
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S-adenosyl-L-homocysteine
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+
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H(+)
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Enzyme class 3:
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Chains Q, R, S, T:
E.C.2.7.7.48
- RNA-directed Rna polymerase.
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Reaction:
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RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
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RNA(n)
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+
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ribonucleoside 5'-triphosphate
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=
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RNA(n+1)
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+
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diphosphate
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Enzyme class 4:
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Chains Q, R, S, T:
E.C.3.4.21.91
- flavivirin.
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Reaction:
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Selective hydrolysis of Xaa-Xaa-|-Xbb bonds in which each of the Xaa can be either Arg or Lys and Xbb can be either Ser or Ala.
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Enzyme class 5:
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Chains Q, R, S, T:
E.C.3.6.1.15
- nucleoside-triphosphate phosphatase.
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Reaction:
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a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H+
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ribonucleoside 5'-triphosphate
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+
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H2O
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=
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ribonucleoside 5'-diphosphate
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+
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phosphate
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+
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H(+)
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Enzyme class 6:
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Chains Q, R, S, T:
E.C.3.6.4.13
- Rna helicase.
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Reaction:
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ATP + H2O = ADP + phosphate + H+
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ATP
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+
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H2O
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=
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ADP
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+
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phosphate
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+
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H(+)
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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J Immunol
188:4971-4979
(2012)
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PubMed id:
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Structural analysis of a dengue cross-reactive antibody complexed with envelope domain III reveals the molecular basis of cross-reactivity.
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C.M.Midgley,
A.Flanagan,
H.B.Tran,
W.Dejnirattisai,
K.Chawansuntati,
A.Jumnainsong,
W.Wongwiwat,
T.Duangchinda,
J.Mongkolsapaya,
J.M.Grimes,
G.R.Screaton.
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ABSTRACT
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Links
