spacer
spacer

PDBsum entry 4aid
Go to PDB code: 
protein ligands Protein-protein interface(s) links
Transferase/peptide PDB id
4aid

 

 

 

 

Loading ...

 
JSmol PyMol  
Contents
Protein chains
573 a.a.
539 a.a.
Ligands
PRO-ARG-ARG-GLY-
TRP-TRP-ARG
LYS-PRO-ARG-ARG-
GLY-TRP-TRP-ARG
ARG-GLY-TRP-TRP-
ARG
PO4 ×3
Waters ×201
PDB id:
4aid
Name: Transferase/peptide
Title: Crystal structure of c. Crescentus pnpase bound to rnase e recognition peptide
Structure: Polyribonucleotide nucleotidyltransferase. Chain: a, b, c. Synonym: pnpase, polynucleotide phosphorylase. Engineered: yes. Ribonuclease, rne/rng family protein. Chain: f, g, h. Fragment: pnpase binding peptide - gww, residues 885-898. Synonym: rnase e. Engineered: yes
Source: Caulobacter vibrioides. Organism_taxid: 190650. Strain: cb15. Atcc: 19089. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 190650
Resolution:
2.60Å     R-factor:   0.210     R-free:   0.254
Authors: S.W.Hardwick,T.Gubbey,I.Hug,U.Jenal,B.F.Luisi
Key ref: S.W.Hardwick et al. (2012). Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly. Open Biol, 2, 120028. PubMed id: 22724061
Date:
09-Feb-12     Release date:   18-Apr-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q9AC32  (PNP_CAUVC) -  Polyribonucleotide nucleotidyltransferase from Caulobacter vibrioides (strain ATCC 19089 / CB15)
Seq:
Struc: 		 
Seq:
Struc: 		712 a.a.
573 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9AC32  (PNP_CAUVC) -  Polyribonucleotide nucleotidyltransferase from Caulobacter vibrioides (strain ATCC 19089 / CB15)
Seq:
Struc: 		 
Seq:
Struc: 		712 a.a.
539 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 14 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, B, C: E.C.2.7.7.8  - polyribonucleotide nucleotidyltransferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: RNA(n+1) + phosphate = RNA(n) + a ribonucleoside 5'-diphosphate
RNA(n+1)
Bound ligand (Het Group name = PO4)
corresponds exactly 		+ phosphate
 = RNA(n)
 + ribonucleoside 5'-diphosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    Added reference    
 
 
Open Biol 2:120028 (2012)
PubMed id: 22724061  
 
 
Crystal structure of Caulobacter crescentus polynucleotide phosphorylase reveals a mechanism of RNA substrate channelling and RNA degradosome assembly.
S.W.Hardwick, T.Gubbey, I.Hug, U.Jenal, B.F.Luisi.
 
  ABSTRACT  
 
No abstract given.

 

 

spacer
spacer