PDBsum entry 4ai1

Cell adhesion

PDB id: 4ai1

Contents

Protein chain

258 a.a.

Ligands

GOL ×2

Metals

_GD ×12

_CL ×2

Waters ×348

PDB id:

4ai1

Name:

Cell adhesion

Title:

Flo5a showing a heptanuclear gadolinium cluster on its surface after 19 min of soaking

Structure:

Flocculation protein flo5. Chain: a. Fragment: lectin-like domain, residues 23-271. Synonym: flocculin-5, flo5a. Engineered: yes. Other_details: soaked 19 min with gdac3

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: s288c. Atcc: 204508. Expressed in: escherichia coli. Expression_system_taxid: 83333. Expression_system_variant: origami 2.

Resolution:

1.80Å R-factor: 0.173 R-free: 0.210

Authors:

M.Veelders,L.-O.Essen

Key ref:

M.Veelders and L.O.Essen (2012). Complex gadolinium-oxo clusters formed along concave protein surfaces. Chembiochem, 13, 2187-2190. PubMed id: 22936626

Date:

07-Feb-12 Release date: 03-Oct-12

Protein chain

P38894  (FLO5_YEAST) -  Flocculation protein FLO5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1075 a.a.

Struc: 258 a.a.*

* PDB and UniProt seqs differ at 9 residue positions (black crosses)

Chembiochem 13:2187-2190 (2012)

PubMed id: 22936626

Complex gadolinium-oxo clusters formed along concave protein surfaces.

M.Veelders, L.O.Essen.

ABSTRACT

Protein-bound contrast: The unusual observation of a heptanuclear gadolinium-oxo cluster on the surface of the cell-adhesion protein Flo5A establishes the basis for directed incorporation of poly-lanthanide clusters into biomolecules. The observed gadolinium cluster might serve as a paradigm for the design of protein-based MRI contrast agents.