PDBsum entry 4a8w

Transferase/DNA

PDB id: 4a8w

Contents

Protein chains

664 a.a.

DNA/RNA

Ligands

_DC-_DG

ATP ×3

Metals

_MG ×3

PDB id:

4a8w

Name:

Transferase/DNA

Title:

Non-catalytic ions direct the RNA-dependent RNA polymerase of bacterial dsrna virus phi6 from de novo initiation to elongation

Structure:

RNA-directed RNA polymerase. Chain: a, b, c. Synonym: protein p2, p2 polymerase. Engineered: yes. Mutation: yes. 5'-d( Tp Tp Cp Gp Cp Gp Tp Ap Ap Gp Cp Gp)-3'. Chain: f, g, h. Engineered: yes

Source:

Pseudomonas phage phi6. Organism_taxid: 10879. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630

Resolution:

3.04Å R-factor: 0.211 R-free: 0.243

Authors:

S.Wright,M.M.Poranen,D.H.Bamford,D.I.Stuart,J.M.Grimes

Key ref:

S.Wright et al. (2012). Noncatalytic ions direct the RNA-dependent RNA polymerase of bacterial double-stranded RNA virus ϕ6 from de novo initiation to elongation. J Virol, 86, 2837-2849. PubMed id: 22205747

Date:

21-Nov-11 Release date: 04-Jul-12

Protein chains

P11124  (RDRP_BPPH6) -  RNA-directed RNA polymerase from Pseudomonas phage phi6

Seq: 665 a.a.

Struc: 664 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

G-C-G 3 bases

T-T-C-G-C-G 6 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

RNA(n) + ribonucleoside 5'-triphosphate = RNA(n+1) (Bound ligand (Het Group name = ATP) matches with 69.23% similarity) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Virol 86:2837-2849 (2012)

PubMed id: 22205747

Noncatalytic ions direct the RNA-dependent RNA polymerase of bacterial double-stranded RNA virus ϕ6 from de novo initiation to elongation.

S.Wright, M.M.Poranen, D.H.Bamford, D.I.Stuart, J.M.Grimes.

ABSTRACT

No abstract given.