PDBsum entry 4a08

DNA-binding protein/DNA

PDB id: 4a08

Contents

Protein chains

1088 a.a.

353 a.a.

DNA/RNA

Ligands

MES ×2

Metals

_CA ×2

Waters ×16

PDB id:

4a08

Name:

DNA-binding protein/DNA

Title:

Structure of hsddb1-drddb2 bound to a 13 bp cpd-duplex (purine at d-1 position) at 3.0 a resolution (cpd 1)

Structure:

DNA damage-binding protein 1. Chain: a. Synonym: ddb p127 subunit, DNA damage-binding protein a, ddba, damage-specific DNA-binding protein 1, hbv x-associated protein 1, xap-1, uv-damaged DNA-binding factor, uv-damaged DNA-binding protein 1, uv-ddb 1, xpe-binding factor, xpe-bf, xeroderma pigmentosum group e-complementing protein, xpce. Engineered: yes. Mutation: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Expression_system_cell_line: high five. Danio rerio. Zebrafish. Organism_taxid: 7955.

Resolution:

3.00Å R-factor: 0.237 R-free: 0.294

Authors:

A.Scrima,E.S.Fischer,S.Iwai,H.Gut,N.H.Thoma

Key ref:

E.S.Fischer et al. (2011). The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation. Cell, 147, 1024-1039. PubMed id: 22118460

Date:

08-Sep-11 Release date: 30-Nov-11

Protein chain

Q16531  (DDB1_HUMAN) -  DNA damage-binding protein 1 from Homo sapiens

Seq: 1140 a.a.

Struc: 1088 a.a.*

Protein chain

Q2YDS1  (DDB2_DANRE) -  DNA damage-binding protein 2 from Danio rerio

Seq: 496 a.a.

Struc: 353 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

C-G-C-G-A-TTD-G-C-G-C 10 bases

G-G-G-C-G-C-C-C-T-C-G-C-G 13 bases

Cell 147:1024-1039 (2011)

PubMed id: 22118460

The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation.

E.S.Fischer, A.Scrima, K.Böhm, S.Matsumoto, G.M.Lingaraju, M.Faty, T.Yasuda, S.Cavadini, M.Wakasugi, F.Hanaoka, S.Iwai, H.Gut, K.Sugasawa, N.H.Thomä.

ABSTRACT

No abstract given.