PDBsum entry 3zza

Hydrolase

PDB id: 3zza

Contents

Protein chain

181 a.a.

Ligands

G84

Waters ×62

PDB id:

3zza

Name:

Hydrolase

Title:

Crystal structure of 3c protease of coxsackievirus b3 complexed with alpha, beta-unsaturated ethyl ester inhibitor 84

Structure:

3c proteinase. Chain: a. Synonym: polyprotein 3bcd. Engineered: yes

Source:

Human coxsackievirus. Organism_taxid: 12072. Strain: b3. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.80Å R-factor: 0.205 R-free: 0.254

Authors:

J.Tan,K.Anand,J.R.Mesters,R.Hilgenfeld

Key ref:

J.Tan et al. Peptidic alpha, Beta-Unsaturated ethyl esters as inhi of the 3c protease of coxsackie virus b3: crystal structures, Antiviral activities, And resistance muta. To be published, .

Date:

31-Aug-11 Release date: 19-Sep-12

Protein chain

Q5UEA2  (Q5UEA2_9ENTO) -  Genome polyprotein from Coxsackievirus B3

Seq: 2185 a.a.

Struc: 181 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 3: E.C.3.4.22.28 - picornain 3C.
• Reaction: Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 4: E.C.3.4.22.29 - picornain 2A.
• Reaction: Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
• Enzyme class 5: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site