PDBsum entry 3wrs

Replication

PDB id: 3wrs

Contents

Protein chain

248 a.a.

Metals

_CO

Waters ×169

PDB id:

3wrs

Name:

Replication

Title:

Minute virus of mice non-structural protein-1n-terminal nuclease domain reveals a unique zn2+ coordination in the active site pocket and shows a novel mode of DNA recognition at the origin of replication

Structure:

Non-capsid protein ns-1. Chain: a. Fragment: unp residues 1-255. Engineered: yes

Source:

Murine minute virus. Mvm. Organism_taxid: 648235. Strain: mvm prototype. Gene: ns1. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.58Å R-factor: 0.182 R-free: 0.218

Authors:

S.K.Tewary,H.Zhao,L.Tang

Key ref:

S.K.Tewary et al. (2015). Structures of minute virus of mice replication initiator protein N-terminal domain: Insights into DNA nicking and origin binding. Virology, 476, 61-71. PubMed id: 25528417 DOI: 10.1016/j.virol.2014.11.022

Date:

27-Feb-14 Release date: 21-Jan-15

Protein chain

P03134  (NS1_MUMIP) -  Initiator protein NS1 from Murine minute virus (strain MVM prototype)

Seq: 672 a.a.

Struc: 248 a.a.

Enzyme reactions

• Enzyme class 2: E.C.3.1.21.- - ?????
• Enzyme class 3: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.virol.2014.11.022

Virology 476:61-71 (2015)

PubMed id: 25528417

Structures of minute virus of mice replication initiator protein N-terminal domain: Insights into DNA nicking and origin binding.

S.K.Tewary, L.Liang, Z.Lin, A.Lynn, S.F.Cotmore, P.Tattersall, H.Zhao, L.Tang.

ABSTRACT

No abstract given.