PDBsum entry 3vuf

Transferase

PDB id: 3vuf

Contents

Protein chain

488 a.a.

Ligands

ADP

SO4 ×3

Waters ×10

PDB id:

3vuf

Name:

Transferase

Title:

Crystal structure of rice granule bound starch synthase i catalytic domain in complex with adp

Structure:

Granule-bound starch synthase 1, chloroplastic/amyloplastic. Chain: a. Fragment: catalytic domain, unp residues 83-609. Synonym: gbss-i. Engineered: yes

Source:

Oryza sativa japonica group. Japanese rice. Organism_taxid: 39947. Strain: japonica cultivar-group. Gene: waxy, wx, wx-b, os06g0133000, loc_os06g04200, 134p10.7, p0679c08.19. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

3.00Å R-factor: 0.224 R-free: 0.271

Authors:

M.Momma,Z.Fujimoto

Key ref:

M.Momma and Z.Fujimoto (2012). Interdomain disulfide bridge in the rice granule bound starch synthase I catalytic domain as elucidated by X-ray structure analysis. Biosci Biotechnol Biochem, 76, 1591-1595. PubMed id: 22878205

Date:

28-Jun-12 Release date: 05-Sep-12

Protein chain

Q0DEV5  (SSG1_ORYSJ) -  Granule-bound starch synthase 1, chloroplastic/amyloplastic from Oryza sativa subsp. japonica

Seq: 609 a.a.

Struc: 488 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.4.1.242 - NDP-glucose--starch glucosyltransferase.
• Reaction: an NDP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl](n) = [(1->4)-alpha-D- glucosyl](n+1) + a ribonucleoside 5'-diphosphate + H⁺

NDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = NDP (Bound ligand (Het Group name = ADP) matches with 60.71% similarity) + ((1->4)-alpha- D-glucosyl)(n+1)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

Biosci Biotechnol Biochem 76:1591-1595 (2012)

PubMed id: 22878205

Interdomain disulfide bridge in the rice granule bound starch synthase I catalytic domain as elucidated by X-ray structure analysis.

M.Momma, Z.Fujimoto.

ABSTRACT

No abstract given.