PDBsum entry 3vlc

Hydrolase/transport protein

PDB id: 3vlc

Contents

Protein chains

302 a.a.

65 a.a.

Ligands

ADP

PDB id:

3vlc

Name:

Hydrolase/transport protein

Title:

Crystal structure of s. Cerevisiae get3 in the semi open conformation in complex with get1 cytosolic domain at 4.5 angstrom resolution

Structure:

Atpase get3. Chain: a. Synonym: arsenical pump-driving atpase, arsenite-stimulated atpase, golgi to er traffic protein 3, guided entry of tail-anchored proteins 3. Engineered: yes. Golgi to er traffic protein 1. Chain: e. Fragment: unp residues 21-104.

Source:

Saccharomyces cerevisiae. Yeast. Organism_taxid: 4932. Strain: nrrl y-53. Expressed in: escherichia coli. Expression_system_taxid: 562. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: get1, mdm39, ygl020c.

Resolution:

4.50Å R-factor: 0.346 R-free: 0.366

Authors:

K.Kubota,A.Yamagata,S.Fukai

Key ref:

K.Kubota et al. (2012). Get1 stabilizes an open dimer conformation of get3 ATPase by binding two distinct interfaces. J Mol Biol, 422, 366-375. PubMed id: 22684149

Date:

30-Nov-11 Release date: 20-Jun-12

Protein chain

Q12154  (GET3_YEAST) -  ATPase GET3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 354 a.a.

Struc: 302 a.a.*

Protein chain

P53192  (GET1_YEAST) -  Golgi to ER traffic protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 235 a.a.

Struc: 65 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.-.-

J Mol Biol 422:366-375 (2012)

PubMed id: 22684149

Get1 stabilizes an open dimer conformation of get3 ATPase by binding two distinct interfaces.

K.Kubota, A.Yamagata, Y.Sato, S.Goto-Ito, S.Fukai.

ABSTRACT

No abstract given.