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PDBsum entry 3vgh
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PDB id:
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Hydrolase
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Title:
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Crystal structure of glycosyltrehalose trehalohydrolase (e283q) complexed with maltotriosyltrehalose
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Structure:
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Malto-oligosyltrehalose trehalohydrolase. Chain: a. Synonym: mthase, 4-alpha-d-((1->4)-alpha-d-glucano)trehalose trehalohydrolase, glycosyltrehalose trehalohydrolase, gthase, maltooligosyl trehalose trehalohydrolase. Engineered: yes. Mutation: yes
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Source:
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Sulfolobus solfataricus. Organism_taxid: 2287. Gene: trez. Expressed in: pichia jadinii. Expression_system_taxid: 4903.
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Resolution:
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2.60Å
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R-factor:
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0.197
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R-free:
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0.244
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Authors:
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N.Okazaki,T.Tamada,M.D.Feese,M.Kato,Y.Miura,T.Komeda,K.Kobayashi, K.Kondo,R.Kuroki
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Key ref:
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N.Okazaki
et al.
(2012).
Substrate recognition mechanism of a glycosyltrehalose trehalohydrolase from Sulfolobus solfataricus KM1.
Protein Sci,
21,
539-552.
PubMed id:
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Date:
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09-Aug-11
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Release date:
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20-Jun-12
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PROCHECK
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Headers
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References
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Q55088
(TREZ_SACSO) -
Malto-oligosyltrehalose trehalohydrolase from Saccharolobus solfataricus
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Seq:
Struc:
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559 a.a.
554 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.3.2.1.141
- 4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase.
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Reaction:
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Hydrolysis of alpha-(1->4)-D-glucosidic linkage in 4-alpha-D- {(1->4)-alpha-D-glucanosyl}(n) trehalose to yield trehalose and alpha- (1->4)-D-glucan.
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Protein Sci
21:539-552
(2012)
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PubMed id:
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Substrate recognition mechanism of a glycosyltrehalose trehalohydrolase from Sulfolobus solfataricus KM1.
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N.Okazaki,
T.Tamada,
M.D.Feese,
M.Kato,
Y.Miura,
T.Komeda,
K.Kobayashi,
K.Kondo,
M.Blaber,
R.Kuroki.
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ABSTRACT
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