PDBsum entry 3vex

Transferase

PDB id

3vex

Loading ...

Contents

			Protein chain

					566 a.a.

			Ligands

					CA0


					SO4 ×2


					EDO ×3

			Metals

					FE2

			Waters ×707

PDB id:

3vex

Links

Name:

Transferase

Title:

Crystal structure of the o-carbamoyltransferase tobz h14n variant in complex with carbamoyl adenylate intermediate

Structure:

O-carbamoyltransferase tobz. Chain: a. Engineered: yes. Mutation: yes

Source:

Streptoalloteichus tenebrarius. Streptomyces tenebrarius. Organism_taxid: 1933. Strain: dsm40770t. Gene: taca, tobz. Expressed in: streptomyces lividans. Expression_system_taxid: 457428.

Resolution:

1.90Å

R-factor:

0.169

R-free:

0.203

Authors:

C.Parthier,M.T.Stubbs,S.Goerlich,F.Jaenecke

Key ref:

C.Parthier et al. (2012). The O-carbamoyltransferase TobZ catalyzes an ancient enzymatic reaction. Angew Chem Int Ed Engl, 51, 4046-4052. PubMed id: 22383337

Date:

09-Jan-12

Release date:

25-Jan-12

PROCHECK

	Headers

	References

Protein chain

Q70IY1 (TOBZ_STRSD) - nebramycin 5' synthase from Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / JCM 4838 / CBS 697.72 / NBRC 16175 / NCIMB 11028 / NRRL B-12390 / A12253. 1)

Seq: Struc:

Seq: Struc:					570 a.a. 566 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

E.C.6.1.2.2 - nebramycin 5' synthase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	tobramycin + carbamoyl phosphate + ATP + H2O = nebramycin 5' + AMP + phosphate + diphosphate + H⁺
2.	kanamycin A + carbamoyl phosphate + ATP + H2O = 6''-O- carbamoylkanamycin A + AMP + phosphate + diphosphate + H⁺

tobramycin	+	carbamoyl phosphate	+	ATP	+	H2O	=	nebramycin 5' Bound ligand (Het Group name = CA0) matches with 88.46% similarity	+	AMP	+	phosphate	+	diphosphate	+	H(+)

kanamycin A

carbamoyl phosphate

ATP

H2O

6''-O- carbamoylkanamycin A

AMP
Bound ligand (Het Group name = CA0)
matches with 88.46% similarity

phosphate

diphosphate

H(+)

Cofactor:

Fe(3+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Angew Chem Int Ed Engl 51:4046-4052 (2012)
PubMed id: 22383337

The O-carbamoyltransferase TobZ catalyzes an ancient enzymatic reaction.
C.Parthier, S.Görlich, F.Jaenecke, C.Breithaupt, U.Bräuer, U.Fandrich, D.Clausnitzer, U.F.Wehmeier, C.Böttcher, D.Scheel, M.T.Stubbs.

ABSTRACT

No abstract given.