PDBsum entry 3v8h

Transferase

PDB id: 3v8h

Contents

Protein chains

312 a.a.

288 a.a.

Ligands

CIT ×3

EDO ×6

Waters ×1162

PDB id:

3v8h

Name:

Transferase

Title:

Crystal structure of thymidylate synthase from burkholderia thailandensis

Structure:

Thymidylate synthase. Chain: a, b, c, d. Synonym: ts, tsase. Engineered: yes

Source:

Burkholderia thailandensis. Organism_taxid: 271848. Strain: e264/atcc 700388/dsm 13276/cip 106301. Gene: thya, bth_i1680. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.65Å R-factor: 0.177 R-free: 0.203

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

L.Baugh et al. (2013). Combining functional and structural genomics to sample the essential Burkholderia structome. Plos One, 8, e53851. PubMed id: 23382856

Date:

22-Dec-11 Release date: 25-Jan-12

Protein chains

Q2SXY2  (Q2SXY2_BURTA) -  Thymidylate synthase from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)

Seq: 323 a.a.

Struc: 312 a.a.

Protein chains

Q2SXY2  (Q2SXY2_BURTA) -  Thymidylate synthase from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)

Seq: 323 a.a.

Struc: 288 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.2.1.1.45 - thymidylate synthase.
• Pathway: Folate Coenzymes
• Reaction: dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Plos One 8:e53851 (2013)

PubMed id: 23382856

Combining functional and structural genomics to sample the essential Burkholderia structome.

L.Baugh, L.A.Gallagher, R.Patrapuvich, M.C.Clifton, A.S.Gardberg, T.E.Edwards, B.Armour, D.W.Begley, S.H.Dieterich, D.M.Dranow, J.Abendroth, J.W.Fairman, D.Fox, B.L.Staker, I.Phan, A.Gillespie, R.Choi, S.Nakazawa-Hewitt, M.T.Nguyen, A.Napuli, L.Barrett, G.W.Buchko, R.Stacy, P.J.Myler, L.J.Stewart, C.Manoil, W.C.Van Voorhis.

ABSTRACT

No abstract given.