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PDBsum entry 3v8e
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PDB id:
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Hydrolase
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Title:
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Crystal structure of the yeast nicotinamidase pnc1p bound to the inhibitor nicotinaldehyde
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Structure:
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Nicotinamidase. Chain: a, b, c, d, e, f, g. Synonym: nicotine deamidase, namase. Engineered: yes
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Source:
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Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: pnc1, ygl037c. Expressed in: escherichia coli. Expression_system_taxid: 562
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Resolution:
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2.71Å
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R-factor:
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0.182
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R-free:
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0.203
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Authors:
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K.A.Hoadley,B.C.Smith,J.M.Denu,J.L.Keck
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Key ref:
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B.C.Smith
et al.
(2012).
Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae.
Biochemistry,
51,
243-256.
PubMed id:
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Date:
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22-Dec-11
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Release date:
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25-Jan-12
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PROCHECK
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Headers
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References
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P53184
(PNC1_YEAST) -
Nicotinamidase from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Seq:
Struc:
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216 a.a.
216 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.3.5.1.19
- nicotinamidase.
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Reaction:
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nicotinamide + H2O = nicotinate + NH4+
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nicotinamide
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+
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H2O
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=
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nicotinate
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+
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NH4(+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Biochemistry
51:243-256
(2012)
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PubMed id:
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Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae.
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B.C.Smith,
M.A.Anderson,
K.A.Hoadley,
J.L.Keck,
W.W.Cleland,
J.M.Denu.
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ABSTRACT
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