PDBsum entry 3v3l

Ligase

PDB id: 3v3l

Contents

Protein chains

83 a.a.

Ligands

V3L ×2

Waters ×125

PDB id:

3v3l

Name:

Ligase

Title:

Crystal structure of human rnf146 wwe domain in complex with iso- adpribose

Structure:

E3 ubiquitin-protein ligase rnf146. Chain: a, b. Fragment: wwe domain residues 100-184. Synonym: dactylidin, ring finger protein 146. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: rnf146. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.65Å R-factor: 0.153 R-free: 0.181

Authors:

Z.Wang,Z.Cheng,W.Xu

Key ref:

Z.Wang et al. (2012). Recognition of the iso-ADP-ribose moiety in poly(ADP-ribose) by WWE domains suggests a general mechanism for poly(ADP-ribosyl)ation-dependent ubiquitination. Genes Dev, 26, 235-240. PubMed id: 22267412

Date:

13-Dec-11 Release date: 15-Feb-12

Protein chains

Q9NTX7  (RN146_HUMAN) -  E3 ubiquitin-protein ligase RNF146 from Homo sapiens

Seq: 359 a.a.

Struc: 83 a.a.

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine

Genes Dev 26:235-240 (2012)

PubMed id: 22267412

Recognition of the iso-ADP-ribose moiety in poly(ADP-ribose) by WWE domains suggests a general mechanism for poly(ADP-ribosyl)ation-dependent ubiquitination.

Z.Wang, G.A.Michaud, Z.Cheng, Y.Zhang, T.R.Hinds, E.Fan, F.Cong, W.Xu.

ABSTRACT

No abstract given.