PDBsum entry 3v3h

Lyase

PDB id: 3v3h

Contents

Protein chain

258 a.a.

Metals

_CL

_ZN

Waters ×220

PDB id:

3v3h

Name:

Lyase

Title:

Kinetic and structural studies of thermostabilized mutants of hca ii.

Structure:

Carbonic anhydrase 2. Chain: b. Synonym: carbonate dehydratase ii, carbonic anhydrasE C, cac, carbonic anhydrase ii, ca-ii. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ca2. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.85Å R-factor: 0.168 R-free: 0.224

Authors:

C.D.Boone,S.Z.Fisher,R.Mckenna

Key ref:

Z.Fisher et al. (2012). Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II. Protein Eng Des Sel, 25, 347-355. PubMed id: 22691706

Date:

13-Dec-11 Release date: 20-Jun-12

Protein chain

P00918  (CAH2_HUMAN) -  Carbonic anhydrase 2 from Homo sapiens

Seq: 260 a.a.

Struc: 258 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.4.2.1.1 - carbonic anhydrase.
• Reaction: hydrogencarbonate + H⁺ = CO2 + H2O
• Cofactor: Zn(2+)
• Enzyme class 3: E.C.4.2.1.69 - cyanamide hydratase.
• Reaction: urea = cyanamide + H2O

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Protein Eng Des Sel 25:347-355 (2012)

PubMed id: 22691706

Kinetic and structural characterization of thermostabilized mutants of human carbonic anhydrase II.

Z.Fisher, C.D.Boone, S.M.Biswas, B.Venkatakrishnan, M.Aggarwal, C.Tu, M.Agbandje-McKenna, D.Silverman, R.McKenna.

ABSTRACT

No abstract given.