PDBsum entry 3uz3

Ribosome/antibiotic

PDB id: 3uz3

Contents

Protein chains

237 a.a.

205 a.a.

208 a.a.

151 a.a.

101 a.a.

155 a.a.

138 a.a.

127 a.a.

99 a.a.

119 a.a.

125 a.a.

116 a.a.

60 a.a.

88 a.a.

84 a.a.

100 a.a.

72 a.a.

78 a.a.

99 a.a.

25 a.a.

DNA/RNA

Ligands

PAR

Metals

_ZN ×2

_MG ×926

Obsolete entry

PDB id:

3uz3

Name:

Ribosome/antibiotic

Title:

Crystal structure analysis of ribosomal decoding. This entry contains the 30s ribosomal subunit of the first 70s molecule in the asymmetric unit for the near-cognate tRNA-leu complex with paromomycin.

Structure:

16s ribosomal RNA. Chain: a. 30s ribosomal protein s2. Chain: e. 30s ribosomal protein s3. Chain: f. 30s ribosomal protein s4. Chain: g. 30s ribosomal protein s5.

Source:

Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Escherichia coli. Organism_taxid: 1110693. Strain: k12. Synthetic: yes. Other_details: synthetic construct

Resolution:

3.30Å R-factor: 0.199 R-free: 0.237

Authors:

L.Jenner,N.Demeshkina,M.Yusupov,G.Yusupova

Key ref:

N.Demeshkina et al. (2012). A new understanding of the decoding principle on the ribosome. Nature, 484, 256-259. PubMed id: 22437501

Date:

07-Dec-11 Release date: 21-Mar-12

Protein chain

P80371  (RS2_THET8) -  Small ribosomal subunit protein uS2 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 256 a.a.

Struc: 237 a.a.

Protein chain

P80372  (RS3_THET8) -  Small ribosomal subunit protein uS3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 239 a.a.

Struc: 205 a.a.

Protein chain

P80373  (RS4_THET8) -  Small ribosomal subunit protein uS4 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 209 a.a.

Struc: 208 a.a.

Protein chain

Q5SHQ5  (RS5_THET8) -  Small ribosomal subunit protein uS5 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 162 a.a.

Struc: 151 a.a.

Protein chain

Q5SLP8  (RS6_THET8) -  Small ribosomal subunit protein bS6 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 101 a.a.

Struc: 101 a.a.

Protein chain

P17291  (RS7_THET8) -  Small ribosomal subunit protein uS7 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 156 a.a.

Struc: 155 a.a.

Protein chain

Q5SHQ2  (RS8_THET8) -

Protein chain

P80374  (RS9_THET8) -  Small ribosomal subunit protein uS9 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 128 a.a.

Struc: 127 a.a.

Protein chain

Q5SHN7  (RS10_THET8) -  Small ribosomal subunit protein uS10 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 105 a.a.

Struc: 99 a.a.

Protein chain

P80376  (RS11_THET8) -  Small ribosomal subunit protein uS11 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 129 a.a.

Struc: 119 a.a.

Protein chain

Q5SHN3  (RS12_THET8) -  Small ribosomal subunit protein uS12 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 132 a.a.

Struc: 125 a.a.

Protein chain

P80377  (RS13_THET8) -  Small ribosomal subunit protein uS13 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 126 a.a.

Struc: 116 a.a.

Protein chain

Q5SHQ1  (RS14Z_THET8) -

Protein chain

Q5SJ76  (RS15_THET8) -  Small ribosomal subunit protein uS15 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 89 a.a.

Struc: 88 a.a.

Protein chain

Q5SJH3  (RS16_THET8) -  Small ribosomal subunit protein bS16 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 88 a.a.

Struc: 84 a.a.

Protein chain

Q5SHP7  (RS17_THET8) -

Protein chain

Q5SLQ0  (RS18_THET8) -  Small ribosomal subunit protein bS18 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 88 a.a.

Struc: 72 a.a.

Protein chain

Q5SHP2  (RS19_THET8) -  Small ribosomal subunit protein uS19 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 93 a.a.

Struc: 78 a.a.

Protein chain

P80380  (RS20_THET8) -  Small ribosomal subunit protein bS20 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 106 a.a.

Struc: 99 a.a.

Protein chain

Q5SIH3  (RSHX_THET8) -  Small ribosomal subunit protein bTHX from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: 27 a.a.

Struc: 25 a.a.

DNA/RNA chains

U-G-G-A-G-A-G-U-U-U-G-A-U-C-C-U-G-G-C-U-C-A-G-G-G-U-G-A-A-C-G-C-U-G-G-C-G-G-C- ... 1506 bases

G-C-C-G-A-G-G-U-G-G-U-G-G-A-A-U-U-G-G-U-A-G-A-C-A-C-G-C-U-A-C-C-U-U-G-A-G-G-U- 87 bases

C-G-C-G-G-G-G-U-G-G-A-G-C-A-G-C-C-C-G-G-U-A-G-C-U-C-G-U-C-G-G-G-C-U-C-A-U-A-A- 77 bases

C-G-C-G-G-G-G-U-G-G-A-G-C-A-G-C-C-C-G-G-U-A-G-C-U-C-G-U-C-G-G-G-C-U-C-A-U-A-A- 77 bases

U-U-U-A-U-G-U-U-U-U 10 bases

Nature 484:256-259 (2012)

PubMed id: 22437501

A new understanding of the decoding principle on the ribosome.

N.Demeshkina, L.Jenner, E.Westhof, M.Yusupov, G.Yusupova.

ABSTRACT

No abstract given.