PDBsum entry 3upm

Hydrolase

PDB id

3upm

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Contents

			Protein chains

					327 a.a.

			Metals

					_CO ×4

			Waters ×439

PDB id:

3upm

Links

Name:

Hydrolase

Title:

Crystal structure of pte mutant h254q/h257f/k185r/i274n

Structure:

Parathion hydrolase. Chain: a, b. Fragment: unp residues 35-361. Synonym: phosphotriesterase, pte. Engineered: yes. Mutation: yes

Source:

Brevundimonas diminuta. Organism_taxid: 293. Gene: opd. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.95Å

R-factor:

0.201

R-free:

0.232

Authors:

P.Tsai,N.G.Fox,Y.Li,D.P.Barondeau,F.M.Raushel

Key ref:

P.C.Tsai et al. (2012). Enzymes for the homeland defense: optimizing phosphotriesterase for the hydrolysis of organophosphate nerve agents. Biochemistry, 51, 6463-6475. PubMed id: 22809162

Date:

18-Nov-11

Release date:

01-Aug-12

PROCHECK

	Headers

	References

Protein chains

P0A434 (OPD_BREDI) - Parathion hydrolase from Brevundimonas diminuta

Seq: Struc:					365 a.a. 327 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 5 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.3.1.8.1 - aryldialkylphosphatase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol

aryl dialkyl phosphate	+	H2O	=	dialkyl phosphate	+	aryl alcohol

Cofactor:

Divalent cation

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	Biochemistry 51:6463-6475 (2012)
PubMed id: 22809162

Enzymes for the homeland defense: optimizing phosphotriesterase for the hydrolysis of organophosphate nerve agents.
P.C.Tsai, N.Fox, A.N.Bigley, S.P.Harvey, D.P.Barondeau, F.M.Raushel.

ABSTRACT

No abstract given.