PDBsum entry 3uo9

Hydrolase/hydrolase inhibitor

PDB id: 3uo9

Contents

Protein chain

411 a.a.

Ligands

GOL ×4

04A ×2

Waters ×531

PDB id:

3uo9

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of human gac in complex with glutamate and bptes

Structure:

Glutaminase kidney isoform, mitochondrial. Chain: a, b, c, d. Fragment: unp residues 71-598. Synonym: gls, k-glutaminase, l-glutamine amidohydrolase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gls, gls1, kiaa0838. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Resolution:

2.30Å R-factor: 0.198 R-free: 0.249

Authors:

B.Delabarre,S.Gross,F.Cheng,Y.Gao,A.Jha,F.Jiang,J.J.Song,W.Wei, J.B.Hurov

Key ref:

B.DeLaBarre et al. (2011). Full-length human glutaminase in complex with an allosteric inhibitor. Biochemistry, 50, 10764-10770. PubMed id: 22049910

Date:

16-Nov-11 Release date: 07-Dec-11

Protein chains

O94925  (GLSK_HUMAN) -  Glutaminase kidney isoform, mitochondrial from Homo sapiens

Seq: 669 a.a.

Struc: 411 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.5.1.2 - glutaminase.
• Reaction: L-glutamine + H2O = L-glutamate + NH4⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Biochemistry 50:10764-10770 (2011)

PubMed id: 22049910

Full-length human glutaminase in complex with an allosteric inhibitor.

B.DeLaBarre, S.Gross, C.Fang, Y.Gao, A.Jha, F.Jiang, J.Song J, W.Wei, J.B.Hurov.

ABSTRACT

No abstract given.