PDBsum entry 3ujf

Lyase

PDB id: 3ujf

Contents

Protein chains

433 a.a.

Ligands

2PG

PEP

Metals

_MG ×4

Waters ×190

PDB id:

3ujf

Name:

Lyase

Title:

Asymmetric complex of human neuron specific enolase-4-pga/pep

Structure:

Gamma-enolase. Chain: a, b. Synonym: 2-phospho-d-glycerate hydro-lyase, enolase 2, neural enolase, neuron-specific enolase, nse. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: eno2. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.197 R-free: 0.259

Authors:

J.Qin,G.Chai,J.Brewer,L.Lovelace,L.Lebioda

Key ref:

J.Qin et al. (2012). Structures of asymmetric complexes of human neuron specific enolase with resolved substrate and product and an analogous complex with two inhibitors indicate subunit interaction and inhibitor cooperativity. J Inorg Biochem, 111, 187-194. PubMed id: 22437160

Date:

07-Nov-11 Release date: 22-Aug-12

Protein chains

P09104  (ENOG_HUMAN) -  Gamma-enolase from Homo sapiens

Seq: 434 a.a.

Struc: 433 a.a.

Enzyme reactions

• Enzyme class: E.C.4.2.1.11 - phosphopyruvate hydratase.
• Reaction: (2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O

(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O (Bound ligand (Het Group name = 2PG) matches with 90.91% similarity)

• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

J Inorg Biochem 111:187-194 (2012)

PubMed id: 22437160

Structures of asymmetric complexes of human neuron specific enolase with resolved substrate and product and an analogous complex with two inhibitors indicate subunit interaction and inhibitor cooperativity.

J.Qin, G.Chai, J.M.Brewer, L.L.Lovelace, L.Lebioda.

ABSTRACT

No abstract given.