PDBsum entry 3ufx

Ligase

PDB id: 3ufx

Contents

Protein chains

288 a.a.

378 a.a.

Ligands

GDP ×4

Metals

_MN ×4

Waters ×735

PDB id:

3ufx

Name:

Ligase

Title:

Thermus aquaticus succinyl-coa synthetase in complex with gdp-mn2+

Structure:

Succinyl-coa synthetase alpha subunit. Chain: a, d, f, h. Synonym: succinyl-coa ligase alpha subunit. Engineered: yes. Succinyl-coa synthetase beta subunit. Chain: b, e, g, i. Synonym: succinyl-coa ligase beta subunit. Engineered: yes

Source:

Thermus aquaticus. Organism_taxid: 271. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

2.35Å R-factor: 0.206 R-free: 0.245

Authors:

M.E.Fraser

Key ref:

M.A.Joyce et al. (2012). Biochemical and structural characterization of the GTP-preferring succinyl-CoA synthetase from Thermus aquaticus. Acta Crystallogr D Biol Crystallogr, 68, 751-762. PubMed id: 22751660

Date:

01-Nov-11 Release date: 27-Jun-12

Protein chains

P09143  (SUCD_THETH) -  Succinate--CoA ligase [GDP-forming] subunit alpha from Thermus thermophilus

Seq: 288 a.a.

Struc: 288 a.a.*

Protein chains

P25126  (SUCC_THETH) -  Succinate--CoA ligase [GDP-forming] subunit beta from Thermus thermophilus

Seq: 378 a.a.

Struc: 378 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, D, E, F, G, H, I: E.C.6.2.1.4 - succinate--CoA ligase (GDP-forming).
• Pathway: Citric acid cycle
• Reaction: GTP + succinate + CoA = succinyl-CoA + GDP + phosphate

GTP + succinate + CoA = succinyl-CoA (Bound ligand (Het Group name = GDP) corresponds exactly) + GDP + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Acta Crystallogr D Biol Crystallogr 68:751-762 (2012)

PubMed id: 22751660

Biochemical and structural characterization of the GTP-preferring succinyl-CoA synthetase from Thermus aquaticus.

M.A.Joyce, K.Hayakawa, W.T.Wolodko, M.E.Fraser.

ABSTRACT

No abstract given.