PDBsum entry 3uby

Hydrolase/DNA

PDB id: 3uby

Contents

Protein chains

204 a.a.

178 a.a.

DNA/RNA

Waters ×250

PDB id:

3uby

Name:

Hydrolase/DNA

Title:

Crystal structure of human alklyadenine DNA glycosylase in a lower and higher-affinity complex with DNA

Structure:

DNA-3-methyladenine glycosylase. Chain: a, b. Fragment: delta79aag. Synonym: 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, adpg, n-methylpurine-DNA glycosylase. Engineered: yes. DNA (5'-d( Gp Ap Cp Ap Tp Gp (Edc)p Tp Tp Gp Cp Cp T)-3'). Chain: c, d. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mpg, aag, anpg, mid1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes

Resolution:

2.00Å R-factor: 0.222 R-free: 0.265

Authors:

J.W.Setser,G.M.Lingaraju,C.A.Davis,L.D.Samson,C.L.Drennan

Key ref:

J.W.Setser et al. (2012). Searching for DNA lesions: structural evidence for lower- and higher-affinity DNA binding conformations of human alkyladenine DNA glycosylase. Biochemistry, 51, 382-390. PubMed id: 22148158

Date:

25-Oct-11 Release date: 28-Dec-11

Protein chain

P29372  (3MG_HUMAN) -  DNA-3-methyladenine glycosylase from Homo sapiens

Seq: 298 a.a.

Struc: 204 a.a.*

Protein chain

P29372  (3MG_HUMAN) -  DNA-3-methyladenine glycosylase from Homo sapiens

Seq: 298 a.a.

Struc: 178 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

DNA/RNA chains

G-A-C-A-T-G-EDC-T-T-G-C 11 bases

G-A-C-A-T-G-EDC-T 8 bases

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.2.2.21 - DNA-3-methyladenine glycosylase Ii.
• Reaction: Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine, and 7-methyladenine.

Biochemistry 51:382-390 (2012)

PubMed id: 22148158

Searching for DNA lesions: structural evidence for lower- and higher-affinity DNA binding conformations of human alkyladenine DNA glycosylase.

J.W.Setser, G.M.Lingaraju, C.A.Davis, L.D.Samson, C.L.Drennan.

ABSTRACT

No abstract given.