PDBsum entry 3u7c

Lyase

PDB id: 3u7c

Contents

Protein chain

258 a.a.

Ligands

CO2 ×2

BCT

GOL

Metals

_ZN

Waters ×546

PDB id:

3u7c

Name:

Lyase

Title:

Crystal structure of the v143i mutant of human carbonic anhydrase ii

Structure:

Carbonic anhydrase 2. Chain: a. Synonym: carbonate dehydratase ii, carbonic anhydrasE C, cac, carbonic anhydrase ii, ca-ii. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ca2. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

0.93Å R-factor: 0.106 R-free: 0.131

Authors:

D.M.West,C.U.Kim,A.H.Robbins,R.Mckenna

Key ref:

D.M.West et al. Crystal structure of the V143I mutant of human carbon anhydrase ii. To be published, .

Date:

13-Oct-11 Release date: 13-Feb-13

Protein chain

P00918  (CAH2_HUMAN) -  Carbonic anhydrase 2 from Homo sapiens

Seq: 260 a.a.

Struc: 258 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: E.C.4.2.1.1 - carbonic anhydrase.
• Reaction: hydrogencarbonate + H⁺ = CO2 + H2O

hydrogencarbonate + H(+) (Bound ligand (Het Group name = BCT) corresponds exactly) = CO2 (Bound ligand (Het Group name = CO2) corresponds exactly) + H2O

• Cofactor: Zn(2+)
• Enzyme class 3: E.C.4.2.1.69 - cyanamide hydratase.
• Reaction: urea = cyanamide + H2O

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site