PDBsum entry 3u3y

Hydrolase/DNA

PDB id: 3u3y

Contents

Protein chains

220 a.a.

DNA/RNA

Ligands

BU1

Metals

_CA ×2

Waters ×183

PDB id:

3u3y

Name:

Hydrolase/DNA

Title:

Mouse trex1 d200h mutant

Structure:

Three prime repair exonuclease 1. Chain: b, a. Synonym: 3'-5' exonuclease trex1. Engineered: yes. Mutation: yes. 5'-d( Gp Ap Cp G)-3'. Chain: c, d. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: trex1. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: chemically synthesized DNA oligonucleotide

Resolution:

2.28Å R-factor: 0.216 R-free: 0.275

Authors:

S.L.Bailey,S.Harvey,F.W.Perrino,T.Hollis

Key ref:

S.L.Bailey et al. (2012). Defects in DNA degradation revealed in crystal structures of TREX1 exonuclease mutations linked to autoimmune disease. Dna Repair (amst), 11, 65-73. PubMed id: 22071149

Date:

06-Oct-11 Release date: 28-Dec-11

Protein chains

Q91XB0  (TREX1_MOUSE) -  Three-prime repair exonuclease 1 from Mus musculus

Seq: 314 a.a.

Struc: 220 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

G-A-C-G 4 bases

G-A-C-G 4 bases

Enzyme reactions

• Enzyme class: E.C.3.1.11.2 - exodeoxyribonuclease Iii.
• Reaction: Degradation of double-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.

Dna Repair (amst) 11:65-73 (2012)

PubMed id: 22071149

Defects in DNA degradation revealed in crystal structures of TREX1 exonuclease mutations linked to autoimmune disease.

S.L.Bailey, S.Harvey, F.W.Perrino, T.Hollis.

ABSTRACT

No abstract given.