PDBsum entry 3ty5

Transferase

PDB id: 3ty5

Contents

Protein chains

381 a.a.

Ligands

ATP

ADP

PGE

Waters ×213

PDB id:

3ty5

Name:

Transferase

Title:

Crystal structure of c. Thermocellum pnkp ligase domain in complex with atp

Structure:

Polynucleotide 2',3'-cyclic phosphate phosphodiesterase / polynucleotide 5'-hydroxyl-kinase / polynucleotide 3'-phosphatase. Chain: a, b. Fragment: nucleotide ligase. Engineered: yes. Mutation: yes

Source:

Clostridium thermocellum. Organism_taxid: 203119. Strain: atcc 27405. Gene: cthe_2768. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.40Å R-factor: 0.180 R-free: 0.225

Authors:

P.Smith,L.Wang,S.Shuman

Key ref:

P.Smith et al. (2012). The adenylyltransferase domain of bacterial Pnkp defines a unique RNA ligase family. Proc Natl Acad Sci U S A, 109, 2296-2301. PubMed id: 22308407

Date:

23-Sep-11 Release date: 25-Jan-12

Protein chains

A3DJ38  (A3DJ38_CLOTH) -  Metallophosphoesterase from Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)

Seq: 870 a.a.

Struc: 381 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.6.5.1.3 - Rna ligase (ATP).
• Reaction: ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate

ATP (Bound ligand (Het Group name = ATP) corresponds exactly) + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Proc Natl Acad Sci U S A 109:2296-2301 (2012)

PubMed id: 22308407

The adenylyltransferase domain of bacterial Pnkp defines a unique RNA ligase family.

P.Smith, L.K.Wang, P.A.Nair, S.Shuman.

ABSTRACT

No abstract given.