PDBsum entry 3ttc

Transferase

PDB id: 3ttc

Contents

Protein chain

646 a.a.

Ligands

ADP ×2

Metals

_ZN ×3

_MG

Waters ×918

PDB id:

3ttc

Name:

Transferase

Title:

Crystal structure of e. Coli hypf with adp and carbamoyl phosphate

Structure:

Transcriptional regulatory protein. Chain: a. Fragment: unp residues 92-746. Synonym: hypf. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 83334. Strain: o157. Gene: ecs3568, hypf. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.86Å R-factor: 0.161 R-free: 0.202

Authors:

S.Petkun,R.Shi,Y.Li,M.Cygler

Key ref:

S.Petkun et al. (2011). Structure of hydrogenase maturation protein HypF with reaction intermediates shows two active sites. Structure, 19, 1773-1783. PubMed id: 22153500

Date:

14-Sep-11 Release date: 28-Dec-11

Protein chain

A0A0H3JHT3  (A0A0H3JHT3_ECO57) -  Carbamoyltransferase HypF from Escherichia coli O157:H7

Seq: 750 a.a.

Struc: 646 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.3.6.1.7 - acylphosphatase.
• Reaction: an acyl phosphate + H2O = a carboxylate + phosphate + H⁺
• Enzyme class 3: E.C.6.2.-.-

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Structure 19:1773-1783 (2011)

PubMed id: 22153500

Structure of hydrogenase maturation protein HypF with reaction intermediates shows two active sites.

S.Petkun, R.Shi, Y.Li, A.Asinas, C.Munger, L.Zhang, M.Waclawek, B.Soboh, R.G.Sawers, M.Cygler.

ABSTRACT

No abstract given.