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PDBsum entry 3t5k
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DNA/transferase
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PDB id
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3t5k
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PDB id:
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| Name: |
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DNA/transferase
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Title:
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Ternary complex of hne adduct modified DNA (5'-txg-3' vs 14-mer) with dpo4 and incoming ddtp
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Structure:
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DNA polymerase iv. Chain: a. Synonym: pol iv. Engineered: yes. DNA (5'-d( Cp Ap Tp (Hn0) p Gp Ap Ap Tp Cp Cp Tp Tp Cp Cp Cp Cp C)-3'). Chain: b. Engineered: yes. Other_details: modified DNA template.
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Source:
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Sulfolobus solfataricus p2. Organism_taxid: 273057. Strain: atcc 35092 / dsm 1617 / jcm 11322 / p2. Gene: dbh, dpo4, sso2448. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthetic construct. Other_details: synthetic construct
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Resolution:
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2.90Å
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R-factor:
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0.212
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R-free:
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0.263
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Authors:
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S.Banerjee,P.P.Christov,A.Kozekova,C.J.Rizzo,M.P.Stone
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Key ref:
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S.Banerjee
et al.
(2012).
Replication bypass of the trans-4-Hydroxynonenal-derived (6S,8R,11S)-1,N(2)-deoxyguanosine DNA adduct by the sulfolobus solfataricus DNA polymerase IV.
Chem Res Toxicol,
25,
422-435.
PubMed id:
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Date:
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27-Jul-11
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Release date:
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15-Feb-12
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PROCHECK
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Headers
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References
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Q97W02
(DPO4_SULSO) -
DNA polymerase IV from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
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Seq:
Struc:
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352 a.a.
341 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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C-A-T-HN0-G-A-A-T-C-C-T-T-C-C-C-C-C
17 bases
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G-G-G-G-G-A-A-G-G-A-T-T-C-C
14 bases
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Enzyme class:
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E.C.2.7.7.7
- DNA-directed Dna polymerase.
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Reaction:
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DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
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DNA(n)
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+
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2'-deoxyribonucleoside 5'-triphosphate
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=
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DNA(n+1)
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+
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diphosphate
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Chem Res Toxicol
25:422-435
(2012)
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PubMed id:
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Replication bypass of the trans-4-Hydroxynonenal-derived (6S,8R,11S)-1,N(2)-deoxyguanosine DNA adduct by the sulfolobus solfataricus DNA polymerase IV.
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S.Banerjee,
P.P.Christov,
A.Kozekova,
C.J.Rizzo,
M.Egli,
M.P.Stone.
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ABSTRACT
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Links
