PDBsum entry 3sky

Hydrolase

PDB id: 3sky

Contents

Protein chain

261 a.a.

Ligands

PO4

MPO

Waters ×92

PDB id:

3sky

Name:

Hydrolase

Title:

2.1a crystal structure of the phosphate bound atp binding domain of archaeoglobus fulgidus copb

Structure:

Copper-exporting p-type atpase b. Chain: a. Fragment: atp binding domain (unp residues 372-636). Engineered: yes

Source:

Archaeoglobus fulgidus. Organism_taxid: 224325. Strain: dsm 4304. Gene: af_0152, copb. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.217 R-free: 0.264

Authors:

S.Jayakanthan,S.A.Roberts,A.Weichsel,J.M.Arguello,M.M.Mcevoy

Key ref:

S.Jayakanthan et al. (2012). Conformations of the apo-, substrate-bound and phosphate-bound ATP-binding domain of the Cu(II) ATPase CopB illustrate coupling of domain movement to the catalytic cycle. Biosci Rep, 32, 443-453. PubMed id: 22663904

Date:

23-Jun-11 Release date: 20-Jun-12

Protein chain

O30085  (COPB_ARCFU) -  Copper-exporting P-type ATPase B from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)

Seq: 690 a.a.

Struc: 261 a.a.

Enzyme reactions

• Enzyme class: E.C.7.2.2.9 - P-type Cu(2+) transporter.
• Reaction: Cu²⁺(in) + ATP + H2O = Cu²⁺(out) + ADP + phosphate + H⁺

Cu(2+)(in) + ATP + H2O = Cu(2+)(out) + ADP + phosphate + H(+) (Bound ligand (Het Group name = PO4) corresponds exactly)

• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biosci Rep 32:443-453 (2012)

PubMed id: 22663904

Conformations of the apo-, substrate-bound and phosphate-bound ATP-binding domain of the Cu(II) ATPase CopB illustrate coupling of domain movement to the catalytic cycle.

S.Jayakanthan, S.A.Roberts, A.Weichsel, J.M.Argüello, M.M.McEvoy.

ABSTRACT

No abstract given.