PDBsum entry 3sgi

Ligase

PDB id: 3sgi

Contents

Protein chain

408 a.a.

Ligands

AMP

Waters ×23

PDB id:

3sgi

Name:

Ligase

Title:

Crystal structure of DNA ligase a brct domain deleted mutant of mycobacterium tuberculosis

Structure:

DNA ligase. Chain: a. Synonym: polydeoxyribonucleotide synthase [nad+]. Engineered: yes. Mutation: yes

Source:

Mycobacterium tuberculosis. Organism_taxid: 1773. Strain: h37rv. Gene: lig, liga, mt3094, mtv012.28c, rv3014c. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

3.50Å R-factor: 0.312 R-free: 0.322

Authors:

V.Kukshal,R.Ravishankar

Key ref:

V.Kukshal and r.ravishankar Crystal structure of domain deleted mutant of mycobac tuberculosis NAD+ dependent DNA ligase capture the am cofactor in a new state. To be published, .

Date:

15-Jun-11 Release date: 20-Jun-12

Protein chain

P9WNV1  (DNLJ_MYCTU) -  DNA ligase A from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Seq: 691 a.a.

Struc: 408 a.a.

Enzyme reactions

• Enzyme class: E.C.6.5.1.2 - Dna ligase (NAD(+)).
• Reaction: NAD⁺ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta- nicotinamide D-nucleotide

NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP (Bound ligand (Het Group name = AMP) matches with 95.65% similarity) + beta- nicotinamide D-nucleotide

Molecule diagrams generated from .mol files obtained from the KEGG ftp site