PDBsum entry 3run

Hydrolase/antibiotic

PDB id: 3run

Contents

Protein chain

168 a.a.

Ligands

MLU-OMZ-ASN-GHP-GHP-OMY-3FG

BGC-RER

MPD

MRD ×2

PO4

TRS

IPA ×2

Metals

_CL

Waters ×319

PDB id:

3run

Name:

Hydrolase/antibiotic

Title:

New strategy to analyze structures of glycopeptide antibiotic-target complexes

Structure:

Lysozyme. Chain: a. Synonym: endolysin, lysis protein, muramidase. Engineered: yes. Mutation: yes. Vancomycin. Chain: b

Source:

Enterobacteria phage t4. Organism_taxid: 10665. Gene: e. Expressed in: escherichia coli. Expression_system_taxid: 562. Streptomyces orientalis. Organism_taxid: 31958

Resolution:

1.40Å R-factor: 0.149 R-free: 0.180

Authors:

N.J.Economou,T.M.Townsend,P.J.Loll

Key ref:

N.J.Economou et al. (2012). A carrier protein strategy yields the structure of dalbavancin. J Am Chem Soc, 134, 4637-4645. PubMed id: 22352468

Date:

05-May-11 Release date: 06-Jun-12

Protein chain

P00720  (ENLYS_BPT4) -  Endolysin from Enterobacteria phage T4

Seq: 164 a.a.

Struc: 168 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.

J Am Chem Soc 134:4637-4645 (2012)

PubMed id: 22352468

A carrier protein strategy yields the structure of dalbavancin.

N.J.Economou, V.Nahoum, S.D.Weeks, K.C.Grasty, I.J.Zentner, T.M.Townsend, M.W.Bhuiya, S.Cocklin, P.J.Loll.

ABSTRACT

No abstract given.