PDBsum entry 3rdo

Biotin binding protein

PDB id: 3rdo

Contents

Protein chain

129 a.a.

Ligands

BTN

SO4

GOL ×2

Metals

_NI

Waters ×193

PDB id:

3rdo

Name:

Biotin binding protein

Title:

Crystal structure of r7-2 streptavidin complexed with biotin

Structure:

Streptavidin. Chain: a. Fragment: unp residues 37-164. Engineered: yes. Mutation: yes

Source:

Streptomyces avidinii. Organism_taxid: 1895. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.40Å R-factor: 0.152 R-free: 0.171

Authors:

V.N.Malashkevich,M.Magalhaes,C.M.Czecster,R.Guan,M.Levy,S.C.Almo

Key ref:

M.L.Magalhães et al. (2011). Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding. Protein Sci, 20, 1145-1154. PubMed id: 21520321

Date:

01-Apr-11 Release date: 06-Jul-11

Protein chain

P22629  (SAV_STRAV) -  Streptavidin from Streptomyces avidinii

Seq: 183 a.a.

Struc: 129 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

Protein Sci 20:1145-1154 (2011)

PubMed id: 21520321

Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding.

M.L.Magalhães, C.M.Czekster, R.Guan, V.N.Malashkevich, S.C.Almo, M.Levy.

ABSTRACT

No abstract given.