PDBsum entry 3r3f

Hydrolase

PDB id: 3r3f

Contents

Protein chains

140 a.a.

Ligands

4CO ×2

Waters ×270

PDB id:

3r3f

Name:

Hydrolase

Title:

Crystal structure of arthrobacter sp. Strain su 4-hydroxybenzoyl coa thioesterase mutant t77a complexed with 4-hydroxyphenacyl coa

Structure:

4-hydroxybenzoyl-coa thioesterase. Chain: a, b. Synonym: 4-hba-coa thioesterase. Engineered: yes. Mutation: yes

Source:

Arthrobacter sp.. Organism_taxid: 1667. Gene: fcbc, fcbc1. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.75Å R-factor: 0.196 R-free: 0.233

Authors:

H.M.Holden,J.B.Thoden,F.Song,Z.Zhuang,M.Trujillo,D.Dunaway-Mariano

Key ref:

F.Song et al. (2012). The catalytic mechanism of the hotdog-fold enzyme superfamily 4-hydroxybenzoyl-CoA thioesterase from Arthrobacter sp. strain SU. Biochemistry, 51, 7000-7016. PubMed id: 22873756

Date:

15-Mar-11 Release date: 28-Mar-12

Protein chains

Q04416  (4HBT_ARTSP) -  4-hydroxybenzoyl-CoA thioesterase from Arthrobacter sp

Seq: 151 a.a.

Struc: 140 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.1.2.23 - 4-hydroxybenzoyl-CoA thioesterase.
• Reaction: 4-hydroxybenzoyl-CoA + H2O = 4-hydroxybenzoate + CoA + H⁺

4-hydroxybenzoyl-CoA (Bound ligand (Het Group name = 4CO) matches with 91.67% similarity) + H2O = 4-hydroxybenzoate + CoA + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochemistry 51:7000-7016 (2012)

PubMed id: 22873756

The catalytic mechanism of the hotdog-fold enzyme superfamily 4-hydroxybenzoyl-CoA thioesterase from Arthrobacter sp. strain SU.

F.Song, J.B.Thoden, Z.Zhuang, J.Latham, M.Trujillo, H.M.Holden, D.Dunaway-Mariano.

ABSTRACT

No abstract given.