PDBsum entry 3r1h

RNA binding protein/RNA

PDB id: 3r1h

Contents

Protein chain

92 a.a.

DNA/RNA

Metals

_CA ×26

Waters ×39

PDB id:

3r1h

Name:

RNA binding protein/RNA

Title:

Crystal structure of the class i ligase ribozyme-substrate preligation complex, c47u mutant, ca2+ bound

Structure:

U1 small nuclear ribonucleoprotein a. Chain: a, d. Fragment: RNA binding domain (unp residues 1-98). Synonym: u1a, u1 snrnp a, u1-a. Engineered: yes. Mutation: yes. 5'-r( Up Cp Cp Ap Gp Up A)-3'. Chain: b, e. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: snrpa. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: RNA was prepared by in vitro transcription with t7 RNA polymerase from linearized plasmid p307hu_c47u

Resolution:

3.15Å R-factor: 0.212 R-free: 0.255

Authors:

D.M.Shechner,D.P.Bartel

Key ref:

D.M.Shechner and D.P.Bartel (2011). The structural basis of RNA-catalyzed RNA polymerization. Nat Struct Biol, 18, 1036-1042. PubMed id: 21857665

Date:

10-Mar-11 Release date: 31-Aug-11

Protein chains

P09012  (SNRPA_HUMAN) -  U1 small nuclear ribonucleoprotein A from Homo sapiens

Seq: 282 a.a.

Struc: 92 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

U-C-C-A-G-U-A 7 bases

GTP-G-A-A-C-A-C-U-A-U-A-C-U-A-C-U-G-G-A-U-A-A-U-C-A-A-A-G-A-C-A-A-A-U-C-U-G-C- 130 bases

U-C-C-A-G-U-A 7 bases

GTP-G-A-A-C-A-C-U-A-U-A-C-U-A-C-U-G-G-A-U-A-A-U-C-A-A-A-G-A-C-A-A-A-U-C-U-G-C- 130 bases

Enzyme reactions

• Enzyme class: E.C.?

Nat Struct Biol 18:1036-1042 (2011)

PubMed id: 21857665

The structural basis of RNA-catalyzed RNA polymerization.

D.M.Shechner, D.P.Bartel.

ABSTRACT

No abstract given.