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PDBsum entry 3qrf
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protein dna_rna metals Protein-protein interface(s) links
DNA binding protein/DNA PDB id
3qrf

 

 

 

 

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Contents
Protein chains
286 a.a.
82 a.a.
DNA/RNA
Metals
_MG ×4
Waters ×7
PDB id:
3qrf
Name: DNA binding protein/DNA
Title: Structure of a domain-swapped foxp3 dimer
Structure: Nuclear factor of activated t-cells, cytoplasmic 2. Chain: n, m. Fragment: human nfat1 DNA binding domain. Synonym: nf-atc2, nfatc2, nfat pre-existing subunit, nf-atp, t-cell transcription factor nfat1. Engineered: yes. Forkhead box protein p3. Chain: f, g, h, i. Fragment: human foxp3 DNA binding domain.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: nfatc2, nfat1, nfatp. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: foxp3, ipex, jm2. Synthetic: yes. Other_details: the core harre2 site occurs naturally in humans..
Resolution:
2.80Å     R-factor:   0.248     R-free:   0.283
Authors: H.S.Bandukwala,Y.Wu,M.Feurer,Y.Chen,B.Barbosa,S.Ghosh,J.C.Stroud, C.Benoist,D.Mathis,A.Rao,L.Chen
Key ref: H.S.Bandukwala et al. (2011). Structure of a domain-swapped FOXP3 dimer on DNA and its function in regulatory T cells. Immunity, 34, 479-491. PubMed id: 21458306
Date:
17-Feb-11     Release date:   20-Apr-11    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q13469  (NFAC2_HUMAN) -  Nuclear factor of activated T-cells, cytoplasmic 2 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		925 a.a.
286 a.a.*
Protein chains
Pfam   ArchSchema ?
Q9BZS1  (FOXP3_HUMAN) -  Forkhead box protein P3 from Homo sapiens
Seq:
Struc: 		431 a.a.
82 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 8 residue positions (black crosses)

DNA/RNA chains
  T-T-A-G-G-A-A-A-A-T-T-T-G-T-T-T-C-A-T-A-G 21 bases
  A-A-C-T-A-T-G-A-A-A-C-A-A-A-T-T-T-T-C-C-T 21 bases
  T-T-A-G-G-A-A-A-A-T-T-T-G-T-T-T-C-A-T-A-G 21 bases
  A-A-C-T-A-T-G-A-A-A-C-A-A-A-T-T-T-T-C-C-T 21 bases

 Enzyme reactions 
   Enzyme class: Chains N, F, G, M, H, I: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
Immunity 34:479-491 (2011)
PubMed id: 21458306  
 
 
Structure of a domain-swapped FOXP3 dimer on DNA and its function in regulatory T cells.
H.S.Bandukwala, Y.Wu, M.Feuerer, Y.Chen, B.Barboza, S.Ghosh, J.C.Stroud, C.Benoist, D.Mathis, A.Rao, L.Chen.
 
  ABSTRACT  
 
No abstract given.

 

 

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