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PDBsum entry 3qpy
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PDB id:
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Transferase
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Title:
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Crystal structure of a mutant (k57a) of 3-deoxy-d-manno-octulosonate 8-phosphate synthase (kdo8ps) from neisseria meningitidis
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Structure:
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2-dehydro-3-deoxyphosphooctonate aldolase. Chain: a, b, c, d. Synonym: 3-deoxy-d-manno-octulosonic acid 8-phosphate synthase, kdo- 8-phosphate synthase, kdo 8-p synthase, kdops, phospho-2-dehydro-3- deoxyoctonate aldolase. Engineered: yes. Mutation: yes
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Source:
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Neisseria meningitidis. Organism_taxid: 122586. Strain: mc58. Gene: kdsa, nmb1283. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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1.95Å
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R-factor:
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0.207
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R-free:
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0.252
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Authors:
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T.M.Allison,G.B.Jameson,E.J.Parker
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Key ref:
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T.M.Allison
et al.
(2011).
Targeting the role of a key conserved motif for substrate selection and catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase.
Biochemistry,
50,
3686-3695.
PubMed id:
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Date:
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14-Feb-11
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Release date:
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13-Apr-11
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PROCHECK
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Headers
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References
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Q9JZ55
(KDSA_NEIMB) -
2-dehydro-3-deoxyphosphooctonate aldolase from Neisseria meningitidis serogroup B (strain MC58)
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Seq:
Struc:
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280 a.a.
250 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.2.5.1.55
- 3-deoxy-8-phosphooctulonate synthase.
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Reaction:
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D-arabinose 5-phosphate + phosphoenolpyruvate + H2O = 3-deoxy-alpha-D- manno-2-octulosonate-8-phosphate + phosphate
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D-arabinose 5-phosphate
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+
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phosphoenolpyruvate
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+
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H2O
Bound ligand (Het Group name = )
matches with 45.45% similarity
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=
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3-deoxy-alpha-D- manno-2-octulosonate-8-phosphate
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+
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phosphate
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Biochemistry
50:3686-3695
(2011)
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PubMed id:
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Targeting the role of a key conserved motif for substrate selection and catalysis by 3-deoxy-D-manno-octulosonate 8-phosphate synthase.
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T.M.Allison,
R.D.Hutton,
F.C.Cochrane,
J.A.Yeoman,
G.B.Jameson,
E.J.Parker.
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ABSTRACT
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Links
