PDBsum entry 3ql3

Oxidoreductase

PDB id: 3ql3

Contents

Protein chain

159 a.a.

Ligands

FOL

NAP

Metals

_MN ×2

Waters ×75

PDB id:

3ql3

Name:

Oxidoreductase

Title:

Re-refined coordinates for PDB entry 1rx2

Structure:

Dihydrofolate reductase. Chain: a. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: fola, tmra, b0048, jw0047. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.80Å R-factor: 0.179 R-free: 0.214

Authors:

G.Bhabha,D.C.Ekiert,P.E.Wright,I.A.Wilson

Key ref:

G.Bhabha et al. (2011). A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis. Science, 332, 234-238. PubMed id: 21474759

Date:

02-Feb-11 Release date: 27-Apr-11

Protein chain

P0ABQ4  (DYR_ECOLI) -  Dihydrofolate reductase from Escherichia coli (strain K12)

Seq: 159 a.a.

Struc: 159 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.5.1.3 - dihydrofolate reductase.
• Pathway: Folate Coenzymes
• Reaction: (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH + H⁺

(6S)-5,6,7,8-tetrahydrofolate + NADP(+) (Bound ligand (Het Group name = NAP) corresponds exactly) = 7,8-dihydrofolate (Bound ligand (Het Group name = FOL) corresponds exactly) + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Science 332:234-238 (2011)

PubMed id: 21474759

A dynamic knockout reveals that conformational fluctuations influence the chemical step of enzyme catalysis.

G.Bhabha, J.Lee, D.C.Ekiert, J.Gam, I.A.Wilson, H.J.Dyson, S.J.Benkovic, P.E.Wright.

ABSTRACT

No abstract given.