PDBsum entry 3pw5

Transferase/DNA

PDB id: 3pw5

Contents

Protein chain

342 a.a.

DNA/RNA

Ligands

TTP

AFN

Metals

_CA ×3

Waters ×57

PDB id:

3pw5

Name:

Transferase/DNA

Title:

Ternary complex of aflatoxin b1 adduct modified DNA (afb1-n7-gua) with DNA polymerase iv and incoming dttp

Structure:

DNA polymerase iv. Chain: a. Fragment: unp residues 1-341. Synonym: pol iv. Engineered: yes. DNA (5'-d( Tp Tp Gp Ap Ap Tp Cp Cp Tp Tp Cp Cp Cp Cp C)- 3'). Chain: b. Engineered: yes.

Source:

Sulfolobus solfataricus. Organism_taxid: 2287. Gene: dbh, dpo4, sso2448. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630

Resolution:

3.00Å R-factor: 0.212 R-free: 0.283

Authors:

S.Banerjee

Key ref:

S.Banerjee et al. (2011). Bypass of aflatoxin B1 adducts by the Sulfolobus solfataricus DNA polymerase IV. J Am Chem Soc, 133, 12556-12568. PubMed id: 21790157

Date:

07-Dec-10 Release date: 10-Aug-11

Protein chain

Q97W02  (DPO4_SULSO) -  DNA polymerase IV from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Seq: 352 a.a.

Struc: 342 a.a.

DNA/RNA chains

T-T-G-A-A-T-C-C-T-T-C-C-C-C-C 15 bases

G-G-G-G-G-A-A-G-G-A-T-T-C 13 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Am Chem Soc 133:12556-12568 (2011)

PubMed id: 21790157

Bypass of aflatoxin B1 adducts by the Sulfolobus solfataricus DNA polymerase IV.

S.Banerjee, K.L.Brown, M.Egli, M.P.Stone.

ABSTRACT

No abstract given.