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PDBsum entry 3pmh
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protein ligands Protein-protein interface(s) links
Blood cloting, hydrolase/inhibitor PDB id
3pmh

 

 

 

 

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Contents
Protein chains
36 a.a.
259 a.a.
284 a.a.
Ligands
NAG-NAG-MAN-MAN-
MAN
0G7
NAG
PDB id:
3pmh
Name: Blood cloting, hydrolase/inhibitor
Title: Mechanism of sulfotyrosine-mediated glycoprotein ib interaction with two distinct alpha-thrombin sites
Structure: Thrombin alpha-chain. Chain: a. Synonym: coagulation factor ii. Thrombin beta-chain. Chain: b. Synonym: coagulation factor ii. Platelet glycoprotein ib alpha chain. Chain: g. Fragment: unp residues 17-306.
Source: Homo sapiens. Human. Organism_taxid: 9606. Other_details: from human plasma. Gene: gp1ba, platelet glycoprotein ib alpha. Expressed in: drosophila melanogaster. Expression_system_taxid: 7227
Resolution:
3.20Å     R-factor:   0.220     R-free:   0.263
Authors: K.I.Varughese,R.Celikel
Key ref: A.Zarpellon et al. (2011). Binding of alpha-thrombin to surface-anchored platelet glycoprotein Ib(alpha) sulfotyrosines through a two-site mechanism involving exosite I. Proc Natl Acad Sci U S A, 108, 8628-8633. PubMed id: 21555542
Date:
16-Nov-10     Release date:   01-Jun-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		622 a.a.
36 a.a.
Protein chain
Pfam   ArchSchema ?
P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		622 a.a.
259 a.a.
Protein chain
Pfam   ArchSchema ?
P07359  (GP1BA_HUMAN) -  Platelet glycoprotein Ib alpha chain from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		652 a.a.
284 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, B: E.C.3.4.21.5  - thrombin.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Preferential cleavage: Arg-|-Gly; activates fibrinogen to fibrin and releases fibrinopeptide A and B.

 

 
Proc Natl Acad Sci U S A 108:8628-8633 (2011)
PubMed id: 21555542  
 
 
Binding of alpha-thrombin to surface-anchored platelet glycoprotein Ib(alpha) sulfotyrosines through a two-site mechanism involving exosite I.
A.Zarpellon, R.Celikel, J.R.Roberts, R.A.McClintock, G.L.Mendolicchio, K.L.Moore, H.Jing, K.I.Varughese, Z.M.Ruggeri.
 
  ABSTRACT  
 
No abstract given.

 

 

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