PDBsum entry 3pmf

Hydrolase

PDB id: 3pmf

Contents

Protein chain

129 a.a.

Ligands

THP

Metals

_CA

Waters ×96

PDB id:

3pmf

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease variant delta+phs v23a at cryogenic temperature

Structure:

Thermonuclease. Chain: a. Fragment: deletion unp residues 126-131. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.60Å R-factor: 0.178 R-free: 0.213

Authors:

J.A.Caro,I.Clark,J.L.Schlessman,B.Garcia-Moreno E.,A.Heroux

Key ref:

J.Roche et al. (2012). Cavities determine the pressure unfolding of proteins. Proc Natl Acad Sci U S A, 109, 6945-6950. PubMed id: 22496593

Date:

16-Nov-10 Release date: 16-Nov-11

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 129 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Proc Natl Acad Sci U S A 109:6945-6950 (2012)

PubMed id: 22496593

Cavities determine the pressure unfolding of proteins.

J.Roche, J.A.Caro, D.R.Norberto, P.Barthe, C.Roumestand, J.L.Schlessman, A.E.Garcia, B.E.García-Moreno, C.A.Royer.

ABSTRACT

No abstract given.