PDBsum entry 3plm

Oxidoreductase

PDB id: 3plm

Contents

Protein chain

296 a.a.

Ligands

AZA

N2O

Metals

_NA

_XE ×2

Waters ×191

PDB id:

3plm

Name:

Oxidoreductase

Title:

Urate oxidase under 2.0 mpa / 20 bars pressure of equimolar mixture xenon : nitrous oxide

Structure:

Uricase. Chain: a. Synonym: urate oxidase. Engineered: yes

Source:

Aspergillus flavus. Organism_taxid: 5059. Gene: uaz, uox. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932

Resolution:

1.62Å R-factor: 0.174 R-free: 0.194

Authors:

G.Marassio,N.Colloc'H,T.Prange,J.H.Abraini

Key ref:

G.Marassio et al. (2011). Pressure-response analysis of anesthetic gases xenon and nitrous oxide on urate oxidase: a crystallographic study. Faseb J, 25, 2266-2275. PubMed id: 21421845

Date:

15-Nov-10 Release date: 06-Apr-11

Protein chain

Q00511  (URIC_ASPFL) -  Uricase from Aspergillus flavus

Seq: 302 a.a.

Struc: 295 a.a.

Enzyme reactions

• Enzyme class: E.C.1.7.3.3 - factor independent urate hydroxylase.
• Pathway: AMP Catabolism
• Reaction: urate + O2 + H2O = 5-hydroxyisourate + H2O2

urate + O2 + H2O (Bound ligand (Het Group name = AZA) matches with 76.92% similarity) = 5-hydroxyisourate + H2O2

• Cofactor: Copper

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Faseb J 25:2266-2275 (2011)

PubMed id: 21421845

Pressure-response analysis of anesthetic gases xenon and nitrous oxide on urate oxidase: a crystallographic study.

G.Marassio, T.Prangé, H.N.David, J.S.Santos, L.Gabison, N.Delcroix, J.H.Abraini, N.Colloc'h.

ABSTRACT

No abstract given.