PDBsum entry 3pfh

Transferase

PDB id: 3pfh

Contents

Protein chains

241 a.a. *

Ligands

SAH ×2

T3Q ×2

EDO

Waters ×478

* Residue conservation analysis

PDB id:

3pfh

Name:

Transferase

Title:

X-ray crystal structure the n,n-dimethyltransferase tylm1 from streptomyces fradiae in complex with sah and dtdp-quip3n

Structure:

N-methyltransferase. Chain: a, d. Engineered: yes

Source:

Streptomyces fradiae. Organism_taxid: 1906. Gene: tylm1, tylmi(orf3 ). Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.79Å R-factor: 0.212 R-free: 0.266

Authors:

A.E.Carney,H.M.Holden

Key ref:

A.E.Carney and H.M.Holden (2011). Molecular architecture of TylM1 from Streptomyces fradiae: an N,N-dimethyltransferase involved in the production of dTDP-D-mycaminose. Biochemistry, 50, 780-787. PubMed id: 21142177

Date:

28-Oct-10 Release date: 15-Dec-10

Protein chains

P95748  (TYLM1_STRFR) -  dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase from Streptomyces fradiae

Seq: 255 a.a.

Struc: 241 a.a.

Enzyme reactions

• Enzyme class: E.C.2.1.1.235 - dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose N,N-dimethyltransferase.
• Reaction: dTDP-3-amino-3,6-dideoxy-alpha-D-glucose + 2 S-adenosyl-L-methionine = dTDP-alpha-D-mycaminose + 2 S-adenosyl-L-homocysteine + 2 H⁺

dTDP-3-amino-3,6-dideoxy-alpha-D-glucose + 2 × S-adenosyl-L-methionine = dTDP-alpha-D-mycaminose + 2 × S-adenosyl-L-homocysteine + 2 × H(+) (Bound ligand (Het Group name = SAH) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Biochemistry 50:780-787 (2011)

PubMed id: 21142177

Molecular architecture of TylM1 from Streptomyces fradiae: an N,N-dimethyltransferase involved in the production of dTDP-D-mycaminose.

A.E.Carney, H.M.Holden.

ABSTRACT

No abstract given.