PDBsum entry 3p1h

Hydrolase

PDB id: 3p1h

Contents

Protein chain

129 a.a.

Ligands

THP

Metals

_CA

Waters ×61

PDB id:

3p1h

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease variant delta+phs v23k/i92a at cryogenic temperature

Structure:

Thermonuclease. Chain: a. Fragment: deletion unp residues 126-131. Engineered: yes. Mutation: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.79Å R-factor: 0.184 R-free: 0.231

Authors:

I.A.Clark,J.A.Caro,G.Sue,J.L.Schlessman,B.Garcia-Moreno E,A.Heroux

Key ref:

J.A.Caro et al. Pressure unfolding effects of artificial cavities in proteins.. To be published, .

Date:

30-Sep-10 Release date: 05-Oct-11

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 129 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.